کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1226552 | 968312 | 2011 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer](/preview/png/1226552.png)
Cancer secretomes are a promising source for biomarker discovery. The analysis of cancer secretomes still faces some difficulties mainly related to the intracellular contamination, which hinders the qualification and follow-up validations. This study aimed to establish a high-quality secretome of A549 cells by using the cellular proteome as a reference and to test the merits of this refined secretome for biomarker discovery for non-small cell lung cancer (NSCLC). Using one-dimensional gel electrophoresis followed by liquid-chromatography tandem mass spectrometry, we comprehensively investigated the secretome and the concurrent cellular proteome of A549 cells. A high-quality secretome consisting of 382 proteins was refined from 889 initial secretory proteins. More than 85.3% of proteins were annotated as secreted and 76.8% as extracellular or membrane-bound. The discriminative power of the lung-cancer associated secretome was confirmed by gene expression and serum proteomic data. The elevated level of C4b-binding Protein (C4BP) in NSCLC blood was verified by enzyme-linked immunosorbent assays (ELISA, p = 6.07e-6). Moreover, the serum C4BP level in 89 patients showed a strong association with the clinical staging of NSCLC. Our reference-experiment-driven strategy is simple and widely applicable, and may facilitate the identification of novel promising biomarkers of lung cancer.
Graphical AbstractFigure optionsDownload high-quality image (137 K)Download as PowerPoint slideResearch Highlights
► A high-quality secretome refined by using the cellular proteome as a reference.
► More than 85.3% of proteins were annotated as secreted and 76.8% as extracellular.
► Independent validation datasets confirmed the merits of the final secretome.
► C4BP in sera (n = 89) showed a strong association with clinical staging of NSCLC.
Journal: Journal of Proteomics - Volume 74, Issue 4, 1 April 2011, Pages 528–538