کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1227015 968340 2012 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Floral reversion mechanism in longan (Dimocarpus longan Lour.) revealed by proteomic and anatomic analyses
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Floral reversion mechanism in longan (Dimocarpus longan Lour.) revealed by proteomic and anatomic analyses
چکیده انگلیسی

Two-dimensional gel electrophoresis (2-DE) was used to analyze the proteins related to floral reversion in Dimocarpus longan Lour. Proteins were extracted from buds undergoing the normal process of flowering and from those undergoing floral reversion in three developing stages in D. longan. Differentially expressed proteins were identified from the gels after 2-DE analysis, which were confirmed using matrix-assisted laser desorption/ionization-time of flying-mass spectroscopy and protein database search. A total of 39 proteins, including 18 up-regulated and 21 down-regulated proteins, were classified into different categories, such as energy and substance metabolism, protein translation, secondary metabolism, phytohormone, cytoskeleton structure, regulation, and stress tolerance. Among these, the largest functional class was associated with primary metabolism. Down-regulated proteins were involved in photosynthesis, transcription, and translation, whereas up-regulated proteins were involved in respiration. Decreased flavonoid synthesis and up-regulated GA20ox might be involved in the floral reversion process. Up-regulated 14-3-3 proteins played a role in the regulation of floral reversion in D. longan by responding to abiotic stress. Observations via transmission electron microscopy revealed the ultrastructure changes in shedding buds undergoing floral reversion. Overall, the results provided insights into the molecular basis for the floral reversion mechanism in D. longan.

Figure optionsDownload high-quality image (80 K)Download as PowerPoint slideHighlights
► Differential expressions of proteomes were found in floral reversion buds of longan.
► Imbalance in photosynthesis and respiration might result in floral reversion.
► Decreased flavonoid and up-regulated GA20ox might be involved in floral reversion.
► TEM revealed ultrastructure changes in shedding flower buds during floral reversion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 75, Issue 4, 2 February 2012, Pages 1099–1118
نویسندگان
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