کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1227022 | 968340 | 2012 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Insights into physiological traits of Bifidobacterium animalis subsp. lactis BB-12 through membrane proteome analysis Insights into physiological traits of Bifidobacterium animalis subsp. lactis BB-12 through membrane proteome analysis](/preview/png/1227022.png)
Bifidobacterium animalis subsp. lactis BB-12 is a widely used probiotic strain associated with a variety of health-promoting traits. There is, however, only limited knowledge available regarding the membrane proteome and the proteins involved in oligosaccharide transport in BB-12. We applied two enrichment strategies to improve the identification of membrane proteins from BB-12 cultures grown on glucose and on xylo-oligosaccharides, the latter being an emerging prebiotic substrate recently reported to be fermented by BB-12. Our approach encompassed consecutive steps of detergent- and carbonate-treatment in order to generate inside-out membrane vesicles and to interfere with binding of membrane-associated proteins to the membrane, respectively. Proteins in the enriched membrane fraction and membrane-associated fraction were digested by lysyl endopeptidase and trypsin followed by peptide sequencing by LC-ESI-Q-TOF MS/MS. Ninety of a total of 248 identified unique proteins were predicted to possess transmembrane segments (TMSs), and 56 of these have more than one TMS. Seventy-nine of the identified proteins are annotated to be involved in transport of amino acids, oligosaccharides, inorganic ions, nucleotides, phosphate or exopolysaccharides, or to belong to the F1F0-ATP-synthetase complex and the protein translocation machinery, respectively.
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► Membrane vesicles were obtained from cultures of a probiotic Bifidobacterium grown on glucose or prebiotic oligosaccharide.
► Membrane proteins enrichment achieved by consecutive detergent treatment, carbonate treatment and enzymatic digestion.
► 248 proteins, incl. 90 transmembrane segments-containing proteins were identified by LC–MS/MS.
► These proteins are associated with (among others) nutrient uptake, acid tolerance and bacteria–host interactions.
Journal: Journal of Proteomics - Volume 75, Issue 4, 2 February 2012, Pages 1190–1200