کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1227037 968340 2012 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Profiling of gender-specific rat plasma proteins associated with susceptibility or resistance to diet-induced obesity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Profiling of gender-specific rat plasma proteins associated with susceptibility or resistance to diet-induced obesity
چکیده انگلیسی

Obesity-prone (OP) and obesity-resistant (OR) rats with different responses to development of obesity in spite of the same genetic background are useful animal models for searching for markers during the development of obesity. Here, we investigated whether plasma proteins of OP and OR rats may behave in a different way in males and females. We performed a comparative proteomic analysis using 2-DE combined with MALDI-TOF/MS on proteins from OP and OR male and female rats to discover gender-specific rat plasma proteins associated with susceptibility or resistance to diet-induced obesity. A total of 29 proteins showing differential expression between the groups were identified by MALDI-TOF/MS and database searches. These proteins were classified into 4 groups according to their regulation patterns in response to diet and gender. 22 proteins showed significant differences between OP and OR rats in males and/or females (Group I, II, and III) and 7 proteins exhibited only a high fat diet (HFD)-responsive difference in male or female rats (Group IV). In conclusion, the proteins negatively (ITIH3, FGG, TUBB5, and ZAG) or positively (Hp, ITIH4, and RBP) correlated with obesity found in this study could be used for selection of new targets for gender specific-medical treatment of obesity.

Figure optionsDownload high-quality image (62 K)Download as PowerPoint slideHighlights
► Proteomic analysis of plasma proteins from male and female OP and OR rats.
► Total of 29 proteins showing differential expression between the groups.
► Total of 22 proteins showing differences between males and females.
► Detected obesity-related inhibitory and stimulatory markers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 75, Issue 4, 2 February 2012, Pages 1386–1400
نویسندگان
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