کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1227095 968345 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Diversity of anti-haemostatic proteins in the salivary glands of Rhodnius species transmitters of Chagas disease in the greater Amazon
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Diversity of anti-haemostatic proteins in the salivary glands of Rhodnius species transmitters of Chagas disease in the greater Amazon
چکیده انگلیسی

The triatomines in the tribe Rhodniini are the main vectors of the Trypanosoma cruzi to humans in recent outbreaks of acute Chagas disease in the Amazon. These insects dwelling in palm trees do not colonize the human domicile. Their success to transmit the infection relies partially on the efficacy of their salivary gland apparatuses. Here we show the transcriptome of the Rhodnius brethesi and Rhodnius robustus salivary glands, comprising 56 and 122 clusters, respectively. Approximately one third of these clusters are described for the first time. The LC–MS/MS analysis identified 123 and 111 proteins in R. brethesi and R. robustus sialome, respectively. Noteworthy, lipocalin platelet aggregation inhibitors, inositol polyphosphate 5-phosphatases, and Kazal domain proteins, which are essential for the insect's successful acquisition of blood meals, were found in our analysis. Moreover, glutathione S transferase and antigen-5, which play roles in the insect's defense and resistance against insecticide, were also observed.

Figure optionsDownload high-quality image (33 K)Download as PowerPoint slideHighlights
► The genus Rhodnius (Gk rhodon, rose) comprise several species of triatomines dwelling in the Amazon.
► The LC-MS/MS proteomes of Rhodnius sp. show proteins and transcripts; one third of which described here for the first time.
► The proteins secure blood meals, resistance against insecticide, and transmission of Trypanosoma cruzi to humans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 9, 24 August 2011, Pages 1664–1672
نویسندگان
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