Validation of LED spectrofluorimeter for determination of both biodiesel and nontransesterified residual cooking oil in diesel samples

• This paper presents the validation of a LED spectrofluorimeter.
• LED spectrofluorimeter was adequate for quantification of biodiesel and RCO in diesel.
• Validation parameters were determined from the regression lines.
• For the analysis of RCO in diesel, the linear range was from 2 to 20% with detection limit of 2%.
• For the analysis of biodiesel in diesel, the range was from 2 to 45% with detection limit of 3%.

This paper presents the results of the validation of a LED spectrofluorimeter patented for the analysis of biodiesel in diesel and non-transesterified residual cooking oil (RCO) in diesel. Detection limit, quantification limit and sensitivity were determined from the regression lines. The spectrofluorimeter validated in this study was adequate for quantifying the amount of biodiesel in diesel in the range from 2% to 45% (B02–B45) with an R-squared value of 0.9962 and a detection limit of 3%. For the analysis of non-transesterified RCO in diesel, the linear range was from 2% to 20% with an R-squared value of 0.9872 and a detection limit of 2%. The accuracy of the equipment for the analysis of biodiesel in diesel and non-transesterified RCO in diesel was evaluated using Student’s t-test for paired data. With 95% confidence level there was no significant difference between the actual values and those determined by the equipment.

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