کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1231020 | 1495258 | 2013 | 8 صفحه PDF | دانلود رایگان |
• Fengycin adopts a turn conformation in a membrane-mimicking environment.
• Glu1 and 5 are involved in calcium ions binding.
• Calcium ions has an influence on the fengycin conformation.
• Calcium ions modifies the environment of the tyrosine residues.
Fengycin is a natural lipopeptide with antifungal and eliciting properties and able to inhibit the activity of phospholipase A2. A combination of CD, FT-IR, NMR and fluorescence spectroscopic techniques was applied to elucidate its conformation in a membrane-mimicking environment and to investigate the effect of calcium ions on it.We mainly observed that fengycin adopts a turn conformation. Our results showed that calcium ions are bound by the two charged glutamates. The calcium binding has an influence on the fengycin conformation and more particularly, on the environment of the tyrosine residues. The modulation of the fengycin conformation by the environmental conditions may influence its biological properties.
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Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 110, June 2013, Pages 450–457