Chemiluminescence determination of trimetazidine via inducing the aggregation of gold nanoparticles

• A simple and sensitive CL method was developed for the determination of trimetazidine.
• Trimetazidine enhanced the CL signal of N-bromosuccinimide-luminol reaction in the presence of gold nanoparticles.
• The method was successfully applied to the determination of trimetazidine in tablets and in spiked serum samples.
• A possible CL reaction mechanism was suggested.

A simple, rapid and sensitive chemiluminescence (CL) method combined with flow injection analysis was developed for the determination of trimetazidine. Trimetazidine was found to significantly increase the CL signal arising from N-bromosuccinimide-luminol reaction in the presence of gold nanoparticles. The enhanced CL intensity was proportional to trimetazidine concentration in the range of 0.01–5.0 μg/mL, with a limit of detection (3sb) of 6.7 ng/mL. The relative standard deviation was 2.8% for 11 repetitive measurements of 0.1 μg/mL trimetazidine solution. The practicality of the method was evaluated by determining trimetazidine in pharmaceutical formulations and in spiked human serum samples. Moreover, the possible CL reaction mechanism was also discussed.

Trimetazidine induced the aggregation of gold nanoparticles (AuNPs), resulting in signal amplification in luminol-N-bromosuccinimide-AuNPs chemiluminescence system, providing a simple and sensitive detection method for trimetazidine.Figure optionsDownload as PowerPoint slide