کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1231778 | 1495215 | 2016 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Potential benefits of triethylamine as n-electron donor in the estimation of forskolin by electronic absorption and emission spectroscopy Potential benefits of triethylamine as n-electron donor in the estimation of forskolin by electronic absorption and emission spectroscopy](/preview/png/1231778.png)
• Detection and assay of forskolin is mainly done by HPLC. Hence alternative are required.
• One of the alternatives is electronic absorption spectra in presence triethylamine, which enhanced the sensitivity.
• Addition of triethylamine enhanced the sensitivity of assay of forskolin by 3.63 times with the fluorescence technique.
Diterpenoid forskolin was isolated from Coleus forskolii. The electronic absorption and emission studies of forskolin were investigated in various solvents with an aim to improve its detection limits. The two chromophores present in the diterpenoid are not conjugated leading to the poor absorption and emission of UV light. The absorption and fluorescence spectra were solvent specific. In the presence of a monodentate ligand, triethylamine the detection of forskolin is improved by 3.63 times in ethanol with the fluorescence method and 3.36 times in DMSO by the absorption spectral method. The longer wavelength absorption maximum is blue shifted while the lower energy fluorescence maximum is red shifted in the presence of triethylamine. From the wavelength of fluorescence maxima of the exciplex formed between excited forskolin and triethylamine it is concluded that the order of reactivity of hydroxyl groups in the excited state forskolin is in the reverse order to that of the order of the reactivity of hydroxyl groups in its ground state.
Addition of n-electron donor triethylamine enhances the sensitivity of forskolin detection by 3.63 times with fluorescence in ethanol and 3.36 times in DMSO by absorption spectral method.Figure optionsDownload as PowerPoint slide
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 154, 5 February 2016, Pages 84–88