Chemiluminescence determination of folic acid by a flow injection analysis assembly

A flow injection (FI) method is reported for the determination of folic acid by chemiluminescence method. This method is based on the reaction of folic acid with Ru(bipy)32+ and Ce(IV) to produce chemiluminescence. The calibration curve was linear over the range of 2.5 × 10−5–3.1 × 10−7 mol/L with a detection limit of 2.3 × 10−8 mol/L (S/N = 3). The relative standard deviation of 1.0 × 10−6 mol/L folic acid was found 3.5% (n = 11). The influences of potential interfering substances were studied. The recovery was higher than 95.3%. The method was accurate, sensitive, and effective for assay of folic acid. This CL method was successfully applied to the determination of folic acid in pharmaceutical preparations. The mechanism of CL reaction was also studied.

The CL kinetic curve (intensity vs. time) of (a) Ru(bipy)32+–Ce(IV) system; and (b) Ru(bipy)32+–folic acid–Ce(IV) system, Conditions: 1.0 × 10−2 M Ru(bipy)33+, 1.0 M Ce(IV), 1.0 M H2SO4 and 1.0 × 10−6 M FA.Figure optionsDownload as PowerPoint slideHighlights
► The CL method has been propose for the determination of folic acid (FA).
► FA strongly enhances the CL emission of Ru(bipy)32+–Ce(IV) system.
► CL intensity of Ru(bipy)32+–Ce(IV) system was proportional to FA concentration.
► A simple, rapid and sensitive flow-injection CL method has been developed.
► The method was successfully applied to the determination of FA in tablet.