Fluorescence quenching of Rhodamine B base by two amines

Fluorescence quenching of Rhodamine B base (RhB) in DMF solution has been studied at different concentrations of the amine Triethyl amine (TEA) and n-butyl amine (NBA) at room temperature. It has been observed that the fluorescence intensity of RhB decrease with increase in the concentration of the TEA and NBA. It has been observed that the quenching due to amines proceeds via dynamic quenching process. The rate constants for the quenching process have been calculated using Stern–Volmer equation. Time resolved fluorescence study and 1H NMR spectral study have also been carried out and discussed.

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► The interaction of Rhodamine B base with two amines have been studied.
► The chosen aliphatic amines are n-butyl amine and triethyl amine.
► 1H NMR spectral study has also been carried out.
► The complex formation has been proved.