Spectrophotometric determination of pico-molar level of hydrazine by using Alizarin red in water and urine samples

• Alizarin red based chemosensor was developed for detection of pico molar level of hydrazine.
• The sensor shows linear range between 5 pM and 40 mM.
• The LOD is calculated from the linear graph as 0.66 pM.
• The metal ions and anions are not having any interference in the detection of hydrazine.

In this paper, very simple and rapid sensor has been developed for the spectrophotometric determination of pico-molar level of hydrazine using Alizarin red. There was a decrease of optical intensity of the probe in the presence of hydrazine. The LOD is calculated from the linear graph between 5–100 pM as 0.66 pM of hydrazine which is well below the risk level proposed by Agency for Toxic Substance and Disease Registry. The probe selectivity for the detection of hydrazine was tested in the presence of commonly encountered metal ions and anions. The calibration curves showed good linearity for working ranges from 5–100 pM and 0.5–40 mM respectively, with R2 = 0.9911 and 0.9744, indicate the validity of the Beer–Lambert law. The binding constant and the free energy change values are determined by the Benesi–Hildebrand method. Determination of hydrazine in environmental water and human urine samples are successfully performed by the proposed method with the recovery of 100%.

Condensation reaction of Alizarin red S with hydrazine.Figure optionsDownload as PowerPoint slide