کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1232188 968787 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence studies of interaction between flavonol p-coumaroylglucoside tiliroside and bovine serum albumin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fluorescence studies of interaction between flavonol p-coumaroylglucoside tiliroside and bovine serum albumin
چکیده انگلیسی

In this paper, the interaction between flavonol p-coumaroylglucoside tiliroside and BSA was investigated by fluorescence quenching spectra, synchronous fluorescence spectra, and three-dimensional fluorescence spectra under simulative physiological conditions. It was proved that the fluorescence quenching of BSA by tiliroside was mainly a result of the formation of a tiliroside–BSA complex. The modified Stern-Volmer quenching constant and the corresponding thermodynamic parameters ΔH, ΔG and ΔS at different temperatures were calculated. The results indicated that electrostatic interactions were the predominant intermolecular forces in stabilizing the complex. The distance r = 3.95 nm between the donor (BSA) and acceptor (tiliroside) was obtained according to Förster's nonradioactive energy transfer theory. The synchronous fluorescence and three-dimensional fluorescence spectra results showed the microenvironment and conformation of BSA were changed in the binding reaction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 77, Issue 2, 1 October 2010, Pages 548–553
نویسندگان
, , ,