کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1232548 968793 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrasensitive determination of serum albumin using resonance light scattering based on ZnS-polyacrylic acid nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Ultrasensitive determination of serum albumin using resonance light scattering based on ZnS-polyacrylic acid nanoparticles
چکیده انگلیسی

ZnS-polyacrylic acid (ZnS-PAA) was prepared by an in situ polymerization method using nano-ZnS as core in the presence of acrylic acid (AA), and ZnS-PAA nanoparticles was characterized by ultraviolet spectrometry (UV) and transmission electron microscopy (TEM). Based on the significant increase of the resonance light scattering (RLS) intensity with the interaction between nanoparticles and serum albumin, RLS method was developed for the sensitive determination of serum albumin (BSA and HSA). Under optimum conditions, the change of the intensity (ΔI) of the RLS spectra at λ = 392 nm was linearly proportional to the concentration of BSA and HSA. The linear range was 1–100 ng mL−1 for HSA and 1–120 ng mL−1 for BSA, and the limit of detection (LOD) was 0.4 ng mL−1 for HSA and 0.5 ng mL−1 for BSA. This method proved to be very sensitive, rapid, simple and tolerant of most interfering substances.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 75, Issue 5, May 2010, Pages 1497–1500
نویسندگان
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