Determination of l-phenylalanine on-line based on molecularly imprinted polymeric microspheres and flow injection chemiluminescence

A novel molecular imprinting-chemiluminescence (MIP-CL) sensor for the determination of l-phenylalanine (Phe) using molecularly imprinted polymer (MIP) as recognition element is reported. The Phe-MIP was synthesized using acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2,2-azobisisobutyronitrile (AIBN) as initiator and the polymers’ properties were characterized. Then the synthesized MIP was employed as recognition element by packing into flow cell to establish a novel flow injection CL sensor. The CL intensity responded linearly to the concentration of Phe in the range 1.3 × 10−6 to 5.44 × 10−4 mol/L with a detection limit of 6.23 × 10−7 mol/L (3σ), which is lower than that of conventional methods. The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the new MIP-CL sensor had been successfully applied to the determination of Phe in samples.

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► The recognition-cell of sensor was placed in front of CL analyzer.
► A switch was used which makes the operation simple in recognition part.
► A straight shape tube filled with MIP for full reaction was used.