A study of Ganoderma lucidum spores by FTIR microspectroscopy

In order to obtain unique information of Ganoderma lucidum spores, FTIR microspectroscopy was used to study G. lucidum spores from Anhui Province (A), Liaoning Province (B) and Shangdong Province (C) of China. IR micro-spectra were acquired with high-resolution and well-reproducibility. The IR spectra of G. lucidum spores from different areas were similar and mainly made up of the absorption bands of polysaccharide, sterols, proteins, fatty acids, etc. The results of curve fitting indicated the protein secondary structures were dissimilar among the above G. lucidum spores. To identify G. lucidum spores from different areas, the H1078/H1640 value might be a potentially useful factor, furthermore FTIR microspectroscopy could realize this identification efficiently with the help of hierarchical cluster analysis. The result indicates FTIR microspectroscopy is an efficient tool for identification of G. lucidum spores from different areas. The result also suggests FTIR microspectroscopy is a potentially useful tool for the study of TCM.

In order to obtain unique information of Ganoderma Lucidum spores, FTIR microspectroscopy was used to study G. Lucidum spores from Anhui Province (A), Liaoning Province (B) and Shangdong Province (C) of China.Figure optionsDownload as PowerPoint slideHighlights
► G. Lucidum spores from three producing areas were studied by FTIR microspectroscopy.
► IR spectra and the secondary derivative IR spectra of G. Lucidum spores were studied.
► Curve fitting was used to study the protein secondary structure of G. Lucidum spores.
► H2956/H2919, H1742/H1640, H1078/H1640 of the above G. Lucidum spores were compared.
► FTIR microspectroscopy can be used to distinguish the above G. Lucidum spores.