کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1233325 | 968806 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Resonance light scattering method for the determination of DNA with cationic methacrylate based polymer nanoparticle probes Resonance light scattering method for the determination of DNA with cationic methacrylate based polymer nanoparticle probes](/preview/png/1233325.png)
Narrowly distributed cationic poly (methyl methacrylate-co-diacetone acrylamide) (P(MMA-DAAM)) nanoparticles were successfully prepared by microemulsion polymerization. Photon correlation spectrometer (PCS) measurement and transmission electron microscope (TEM) observation revealed that z-average particle size of P(MMA-DAAM) is ∼27.5 nm. It was found that these cationic nanoparticles interact with DNA through electrostatic interaction to form P(MMA-DAAM)–DNA complex, which significantly enhances the resonance light scattering (RLS) signal. Therefore, a novel method using this polymer nanoparticle as a new probe for the detection of DNA by RLS technique is developed in this paper. The results showed this method is very convenient, sensitive, and reproducible.
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► P(MMA-DAAM) interact with DNA through electrostatic interaction to form complex.
► Interaction cause obvious change in size, morphology and zeta potential of complex.
► The formation of P(MMA-DAAM)-DNA complex significantly enhances the RLS signal.
► Some co-existing species had little effects on the determination of DNA.
► Our approach for DNA detection has a low determination limit (3σ) of 10.01ng mL-1.
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 82, Issue 1, November 2011, Pages 437–443