Surface-enhanced fluorescent immunoassay on 2D silver nanotriangles array

• Silver nanotriangles array with long ranged periodicity was fabricated on the surface of glass wafer.
• High enhancement of the fluorescent intensity of the target antibodies was obtained by the silver nanotriangles array.
• High sensitivity and good reproducibility for antibodies detection was obtained by the 2D silver nanotriangles array.

Long range ordered silver nanotriangles array was fabricated for surface-enhanced fluorescent immunoassay in this paper. By polystyrene (PS) microspheres based LB template method, the silver nanotriangle array with about 100 nm in height was constructed on the surface of glass slide. On the surface of Ag nanotriangles array, the immune reaction of antigens and labeled antibodies was carried out. Based on the interaction of fluorophores from antibodies with the plasmon resonance from Ag nanotriangles and the enrichment effect of this patterned array, 3.11 times enhancement of the fluorescent intensity of the target antibodies was obtained. According to the fitting curve of fluorescent intensities and logarithmic concentrations of labeled antibodies from 100 pg/mL to 10 μg/mL, it concludes that the limit of detection by this Ag nanotriangles array for immune complex is 100 pg/mL. Due to the advantages of high sensitivity, good reproducibility, and convenient fabrication, the 2D silver nanotriangles array could be an exciting platform for bioassays in proteomics, drug discovery and diagnostics.

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