The influence of fatty acids on theophylline binding to human serum albumin. Comparative fluorescence study

Theophylline, popular diuretic, is used to treat asthma and bronchospasm. In blood it forms complexes with albumin, which is also the main transporter of fatty acids. The aim of the present study was to describe the influence of fatty acids (FA) on binding of theophylline (Th) to human serum albumin (HSA) in the high affinity binding sites. Binding parameters have been obtained on the basis of the fluorescence analysis.The data obtained for the complex of Th and natural human serum albumin (nHSA) obtained from blood of obese patients qualified for surgical removal of stomach was compared with our previous studies on the influence of FA on the complex of Th and commercially available defatted human serum albumin (dHSA).

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► We studied the influence of FA on binding of Th to HSA.
► We used fluorescence techniques.
► We concluded that changes in the affinity of HSA are induced by the presence of FA.
► We showed that pathological factors accompanying obesity contribute in the changes.