Surface-enhanced Raman scattering (SERS) spectra of hemoglobin of mouse and rabbit with self-assembled nano-silver film

The nano-silver film was prepared by electrolysis method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to detect the morphology of the nano-silver particles. The SERS spectra of the hemoglobin (rabbit and mouse) on nano-silver film were gained. It could be known from the SERS spectra that the nano-silver films could enhance the Raman signal of the hemoglobin efficiently, and the sodium citrate and PBS create no influence to the SERS spectra of the hemoglobin. Using this electrolysis technique to fabricate highly bio-active, stable, reusable, and low-cost SERS substrate will be useful in the development of hemoglobin detection.

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► Nano-silver film put as active SERS substrate made by electrolysis method.
► SERS spectra of hemoglobin of mouse and rabbit were obtained on nano-silver film.
► Sodium citrate and PBS made no affection to the OxyHb in SERS experiment.
► Portable Raman Spectrometer associate with nano-silver film has good future.