کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1234925 968839 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study of interactions of anthraquinones with DNA using ethidium bromide as a fluorescence probe
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Study of interactions of anthraquinones with DNA using ethidium bromide as a fluorescence probe
چکیده انگلیسی

The interactions of fish sperm deoxyribonucleic acid (DNA) with anthraquinones, such as chrysophanol, physcion and 1,8-dihydroxy anthraquinone, were investigated by using ethidium bromide (EB) as fluorescence probe. The binding constants of anthraquinones and DNA were obtained by the fluorescence quenching technique. Further, the binding mechanisms on the reaction of the three anthraquinones with DNA and effect of ionic strength on the fluorescence property of the system have also been investigated. The results of the assay indicate that the binding modes of chrysophanol, physcion and 1,8-dihydroxy anthraquinone with DNA were evaluated to be groove binding. And the binding constants of chrysophanol, physcion and 1,8-dihydroxy anthraquinone with DNA–EB complex were 1.64 × 104, 3.04 × 104 and 2.88 × 105 l mol−1, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 70, Issue 1, June 2008, Pages 136–143
نویسندگان
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