Label-free DNA hybridization detection by various spectroscopy methods using triphenylmethane dyes as a probe

► A new assay hybridization using trace of triphenylmethane dye as a probe. ► With the RLS measurement, labeling neither the target nor the probe DNA. ► The actual sample testing with satisfactory results. ► The technique is simple, robust, accurate, can be completed in less than 1 h.