Determining the mode of interaction of calf thymus DNA with the drug sumatriptan using voltammetric and spectroscopic techniques

The interaction of native calf thymus DNA with sumatriptan(1-[3-(2-dimethylaminoethyl)-1H-indol-5-yl]-N-methyl-methanesulfonamide) at physiological pH was studied by spectrophotometry, circular dichroism, voltammetry and viscosimetric techniques. Sumatriptan molecule intercalated between base pairs of DNA, showed by a sharp increase in specific viscosity of DNA. In cyclic voltammetry, decrease of the peak current and positive shift indicated that this drug is able to intercalate between the DNA base pairs. In addition, the drug induced changes in the CD spectrum of CT-DNA, as well as hypochromism changes in its UV–vis spectrum.

In this study, an attempt has been made to study the interaction of sumatriptan with calf thymus DNA it employing UV–vis, voltammetric, viscometric and circular dichroism (CD) techniques.Figure optionsDownload as PowerPoint slideHighlights
► Search for new molecular structures which exhibit affinity for double strand DNA among the popular drugs.
► The DRUG can bind to DNA via intercalative mode.
► Several spectroscopic techniques have been used in this research.