Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity

Biosynthesis of silver nanoparticles (AgNPs) was achieved by a novel, simple green chemistry procedure using citrus sinensis peel extract as a reducing and a capping agent. The effect of temperature on the synthesis of silver nanoparticles was carried out at room temperature (25 °C) and 60 °C. The successful formation of silver nanoparticles has been confirmed by UV–vis, FTIR, XRD, EDAX, FESEM and TEM analysis and their antibacterial activity against Escherichia coli, Pseudomonas aeruginosa (Gram-negative), and Staphylococcus aureus (Gram-positive) has been studied. The results suggest that the synthesized AgNPs act as an effective antibacterial agent.

The zone of inhibition of biosynthesized silver nanoparticles against, (A) Escherichia coli, (B) Pseudomonas aeruginosa and (C) Staphylococcus aureus.Figure optionsDownload as PowerPoint slideHighlights
► A novel approach of synthesis of silver nanoparticles using citrus sinensis peels.
► The peel extract acts as bioreducing and capping agents.
► The silver nanoparticles formed are spherical in shape and 35 and 10 nm of size synthesized at 25 °C and 60 °C, respectively.
► Act as a good antibacterial agent against Gram-negative and Gram-positive bacteria.