کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1236457 | 968870 | 2011 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Spectroscopic studies on interaction and sonodynamic damage of metallochlorophyllin (Chl-M (M = Fe, Zn and Cu)) to protein under ultrasonic irradiation Spectroscopic studies on interaction and sonodynamic damage of metallochlorophyllin (Chl-M (M = Fe, Zn and Cu)) to protein under ultrasonic irradiation](/preview/png/1236457.png)
In this paper, the chlorophyll derivatives, metallochlorophyllin (Chl-M) (M = Fe, Zn and Cu) including chlorophyllin iron (Chl-Fe), chlorophyllin zinc (Chl-Zn) and chlorophyllin copper (Chl-Cu), were adopted as sonosensitizers to combine with ultrasonic irradiation, and the sonodynamic damage of bovine serum albumin (BSA) was investigated. At first, the interaction of Chl-M with BSA was studied by fluorescence spectroscopy. The results show that the quenching mechanism belongs to a static process and among them the affinity of Chl-Fe to BSA is the most obvious. Then, some influence factors on the sonodynamic damage of BSA molecules in the presence of Chl-M under ultrasonic irradiation were also studied. Synchronous fluorescence spectra show that the binding and damage sites of Chl-M to BSA molecule are mainly on the tryptophan (Trp) residues. The generation of ROS in Chl-M sonodynamic process is estimated by the method of Oxidation-Extraction Spectrometry (OEP). This paper may offer some valuable references for the study of the sonodynamic activity of Chl-M and the effect of the central metals. Synchronously, it contributes to the application of Chl-M in SDT for tumor treatment.
The metallochlorophyllin (Chl-M) were adopted as sonosensitizers to combine with ultrasonic irradiation, and the sonodynamic damage of bovine serum albumin (BSA) was investigated. The order of the sonodynamic activities is Chl-Fe > Chl-Zn > Chl-Cu. Synchronous fluorescence spectra show that the damage sites of Chl-M to BSA are mainly on the tryptophan (Trp) residues of BSA. The reactive oxygen species (ROS) generated during sonodynamic process was estimated as 1O2 and OH by the method of Oxidation-Extraction Spectrometry (OEP).Figure optionsDownload as PowerPoint slideHighlights
► The interaction of metallochlorophyllin with BSA was studied.
► The sonodynamic damage of BSA in the presence of Chl-M was studied.
► The formations of ROS during sonocatalytic process were studied.
► The kinds of ROS were determined by adding some scavengers.
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 79, Issue 5, September 2011, Pages 849–857