کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1236466 | 968870 | 2011 | 5 صفحه PDF | دانلود رایگان |
The present work reports on Raman and Surface Enhanced Raman Scattering (SERS) vibrational fingerprints of β-carotene and haemoglobin in fresh whole blood (i.e. right after blood test) with different laser excitations, i.e. visible (514 nm) and near-infrared (NIR, 785 nm). The use of colloidal silver nanoparticles significantly increases the Raman signal, thus providing a clear SERS spectrum of blood. The collected spectra have been examined and marker bands of β-carotene and of the haem prosthetic group of haemoglobin have been found.In particular, the fundamental features of β-carotene (514 nm excitation), blood proteins and haem molecules (785 nm excitation) were recognized and assigned. Moreover haemoglobin SERS signals can be identified and related with its oxygenation state (oxy-haemoglobin). The data reported show the prospects of Raman and SERS techniques to detect important bio-molecules in a whole blood sample with no pre-treatment.
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► Fundamental vibrational fingerprints of β-carotene and haemoglobin have been collected in fresh whole blood, using different laser excitations.
► The use of colloidal silver nanoparticles significantly increases the Raman signal, thus providing a clear SERS spectrum of blood.
► The data reported show the prospects of Raman and SERS techniques to detect important bio-molecules in a whole blood sample with no pre-treatment.
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 79, Issue 5, September 2011, Pages 915–919