Oxidation–extraction spectrometry of reactive oxygen species (ROS) generated by chlorophyllin magnesium (Chl-Mg) under ultrasonic irradiation

In order to examine the mechanism and process of sonodynamic reaction, the chlorophyllin magnesium (Chl-Mg) acting as a sonosensitizer was irradiated by ultrasound, and the generation of reactive oxygen species (ROS) were detected by the method of oxidation–extraction spectrometry (OES). That is, under ultrasonic irradiation in the presence of Chl-Mg, the 1,5-diphenyl carbazide (DPCI) is oxidized by generated ROS into 1,5-diphenyl carbazone (DPCO), which can be extracted by mixed organic solvent and display a obvious visible absorption at 563 nm wavelength. Besides, the generation conditions of ROS were also reviewed. The results demonstrated that the quantities of generated ROS increased with the increase of ultrasonic irradiation time, Chl-Mg concentration and DPCI concentration. Finally, several radical scavengers (l-Histidine (His), 2,6-Di-tert-butyl-methylphenol (BHT) and Vitamin C (VC)) were used to determine the kind of the generated ROS. It was found that at least the hydroxyl radical (OH) and singlet oxygen (1O2) were generated in the presence of Chl-Mg under ultrasonic irradiation. It is wish that this paper might offer some valuable references for the study on the mechanism of SDT and the application of Chl-Mg in tumor treatment.

The generation of reactive oxygen species (ROS) in the presence of chlorophyllin magnesium (Chl-Mg) under ultrasonic irradiation was detected by the method of oxidation–extraction spectrometry (OES). The kind of the generated ROS was determined by using several radical scavengers. The hydroxyl radical (OH) and singlet oxygen (1O2) generated in the sonodynamic process were found at least.Figure optionsDownload as PowerPoint slideHighlights
► ROS were detected by using the method of oxidation–extraction spectrometry (OES).
► The kind of ROS was determined by adding some radical scavengers.
► Some important influences on generation of ROS were studied in sonodynamic process.