Spectrofluorometric studies on the interaction between oxacalix[6]arene-locked trizinc(II)porphyrins and crystal violet

A new fluorogenic oxacalix[6]arene bearing three zinc(II) porphyrin groups (1) was prepared. When crystal violet (CV) was bound to 1, fluorescence intensity of 1 was quenched regularly. This indicates that 1 and CV formed a 1:1 complex and the association constant was calculated. The inclusion mechanism was also discussed. This new fluorometric method was developed for rapid determination of CV with a good linearity in the concentration range of 1.40–37.4 μmol L−1. The method was applied for the determination of CV in synthetic samples successfully.