کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1251955 1496313 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Membrane effects of lysozyme amyloid fibrils
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Membrane effects of lysozyme amyloid fibrils
چکیده انگلیسی

The influence of mature lysozyme fibrils on the structural and physical properties of model membranes composed of phosphatidylcholine (PC) and its mixtures with cardiolipin (CL) (10 mol%) and cholesterol (Chol) (30 mol%) was studied using fluorescent probes DPH, pyrene, Laurdan and MBA. Analysis of pyrene fluorescence spectra along with the measurements of DPH fluorescence anisotropy revealed that the structure of hydrocarbon chains region of lipid bilayer is not affected by the fibrillar aggregates of lysozyme. In contrast, probing the membrane effects by Laurdan and MBA showed the rise of both the generalized polarization of Laurdan and the MBA fluorescence anisotropy, suggesting that amyloid protein induces reduction of bilayer hydration and increase of lipid packing in the interfacial region of model membranes.

Figure optionsDownload as PowerPoint slideHighlights
► Nonpolar region of lipid bilayer is not affected by the fibrillar lysozyme.
► Amyloid fibrils produce bilayer dehydration and/or enhance lipid packing density.
► Cholesterol was found to reduce membrane-modifying propensity of lysozyme fibrils.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 165, Issue 3, April 2012, Pages 331–337
نویسندگان
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