کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1253728 1496357 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of temperature and pH on 31P nuclear magnetic resonances of phospholipids in cholate micelles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Effect of temperature and pH on 31P nuclear magnetic resonances of phospholipids in cholate micelles
چکیده انگلیسی

Accurate and precise determination of phospholipid composition by 31P NMR spectroscopy requires correct assignments and adequate spectral resolution. Because temperature and pH may affect chemical shifts (δ), our first aim was to establish the temperature coefficient (Δδ/ΔT) of common phospholipid classes when using sodium cholate as detergent. This parameter can then be used to aid in resonance assignments. The second goal was to investigate the pH dependence of δ so that, in addition to temperature, pH control can be used to minimize spectral overlap. For phosphatidylcholine, sphingomyelin, dihydrosphingomyelin and phosphatidylglycerol, δ values were invariant with pH and temperature. Whereas the Δδ/ΔT for phosphatidylinositol was 4 × 10−3 ppm/°C, regardless of pH, these coefficients were highly pH-dependent for phosphatidic acid, phosphatidylethanolamine and phosphatidylserine, exhibiting maximal variations with the deprotonation of the headgroup, particularly for phosphatidic acid. These trends indicate the importance of H-bonding on δ and Δδ/ΔT for phospholipid resonances.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 150, Issue 2, December 2007, Pages 176–185
نویسندگان
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