کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1256745 1496489 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reconstitution of cytoskeletal protein assemblies for large-scale membrane transformation
ترجمه فارسی عنوان
بازسازی پروتئین های سیتو اسکلتی برای تبدیل غشای بزرگ مقیاس
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی


• Assays for bottom-up reconstitution of large-scale membrane transformations.
• FtsZ and actin contractile ring structures as common theme for minimal cytokinesis.
• First elementary constriction events in vesicles by bacterial divisome proteins.

Membranes determine two-dimensional and three-dimensional biochemical reaction spaces in living systems. Defining size and shape of surfaces and volumes encompassed by membrane is of key importance for cellular metabolism and homeostasis, and the maintenance and controlled transformation of membrane shapes are coordinated by a large number of different protein assemblies. The orchestration of spatial elements over distances orders of magnitudes larger than protein molecules, as required for cell division, is a particularly challenging task, requiring large-scale ordered protein filaments and networks. The structure and function of these networks, particularly of cytoskeletal elements, have been characterized extensively in cells and reconstituted systems. However, their co-reconstitution with membranes from the bottom-up under defined conditions, to elucidate their mode of action in detail, is still a relatively new field of research. In this short review, we discuss recent approaches and achievements with regard to the study of cytoskeletal protein assemblies on model membranes, with specific focus on contractile elements as those based on the bacterial division FtsZ protein and eukaryotic actomyosin structures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 22, October 2014, Pages 18–26
نویسندگان
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