کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1256886 971535 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening
چکیده انگلیسی

In traditional screening with 96-well plates, microliters of substrates are consumed for each reaction. Further miniaturization is limited by the special equipment and techniques required to dispense nanoliter volumes of fluid. Plug-based microfluidics confines reagents in nanoliter plugs (droplets surrounded by fluorinated carrier fluid), and uses simple pumps to control the flow of plugs. By using cartridges pre-loaded with nanoliter plugs of reagents, only two pumps and a merging junction are needed to set up a screen. Screening with preloaded cartridges uses only nanoliters of substrate per reaction, and requires no microfabrication. The low cost and simplicity of this method has the potential of replacing 96-well and other multi-well plates, and has been applied to enzymatic assays, protein crystallization and optimization of organic reactions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 10, Issue 3, June 2006, Pages 226–231
نویسندگان
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