کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1257265 971553 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single molecule enzymology: watching the reaction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Single molecule enzymology: watching the reaction
چکیده انگلیسی

Single molecule optical microscopy can directly monitor substrate turnover by individual enzymes revealing the underlying distribution of reaction rates and enzyme conformations. These techniques are particularly useful for assessing cooperativity in multi-subunit enzymes such as β-galactosidase, and for directly monitoring how ligand and substrate binding alter dynamic equilibria. Recent investigations of HIV reverse transcriptase have reiterated the importance of single molecule microscopy for determining how proteins move on oligonucleotides and how ligands and inhibitors affect motion. Similar investigations of membrane active enzymes allow direct imaging of protein–membrane interactions. For a large variety of systems, single molecule enzymology provides unprecedented images of how enzymes interact with their substrates and the differences between individual enzymes in a population.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 13, Issue 4, October 2009, Pages 436–442
نویسندگان
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