کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1257540 971567 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Small-molecule catalysts of oxidative protein folding
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Small-molecule catalysts of oxidative protein folding
چکیده انگلیسی

Oxidative protein folding occurs both in vivo and in vitro and involves the formation and rearrangement of protein disulfide bonds (SS bonds). In vivo these reactions are catalyzed by enzymes, including the eukaryotic enzyme protein disulfide isomerase (PDI). Using the physical properties of PDI as a guide, several small-molecule catalysts of oxidative protein folding have been designed, synthesized, and tested. These small molecules can improve the folding rate of the model substrate ribonuclease A by a factor of over 10 and improve the yield by up to a factor of 3 over traditional conditions. The molecules have also been demonstrated to significantly improve the in vivo folding of proteins as well.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 12, Issue 6, December 2008, Pages 740–745
نویسندگان
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