کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1258995 1496481 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-throughput platforms for metabolomics
ترجمه فارسی عنوان
پلتفرم های با کارایی بالا برای متابولومیک
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی


• We review high-throughput mass spectrometry-based methods for metabolomic analysis.
• High-throughput approaches usually improve throughput at the expense of coverage.
• Microfluidic-MS will be able to bridge the gap between throughput and coverage.

Mass spectrometry has become a choice method for broad-spectrum metabolite analysis in both fundamental and applied research. This can range from comprehensive analysis achieved through time-consuming chromatography to the rapid analysis of a few target metabolites without chromatography. In this review article, we highlight current high-throughput MS-based platforms and their potential application in metabolomics. Although current MS platforms can reach throughputs up to 0.5 seconds per sample, the metabolite coverage of these platforms are low compared to low-throughput, separation-based MS methods. High-throughput comes at a cost, as it's a trade-off between sample throughput and metabolite coverage. As we will discuss, promising emerging technologies, including microfluidics and miniaturization of separation techniques, have the potential to achieve both rapid and more comprehensive metabolite analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 30, February 2016, Pages 7–13
نویسندگان
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