کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1258998 1496481 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Advances in discovering small molecules to probe protein function in a systems context
ترجمه فارسی عنوان
پیشرفت در کشف مولکول های کوچک برای بررسی عملکرد پروتئین در زمینه سیستم
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی


• We review selected recent developments in chemical high-throughput screening (HTS).
• Target-based HTS assays have recently been adapted for new target classes.
• Cell-based HTS assays rely on the ability to define and quantify complex phenotypes.
• Both strategies have yielded effective probes for studying uncharacterized biology.

High throughput screening (HTS) has historically been used for drug discovery almost exclusively by the pharmaceutical industry. Due to a significant decrease in costs associated with establishing a high throughput facility and an exponential interest in discovering probes of development and disease associated biomolecules, HTS core facilities have become an integral part of most academic and non-profit research institutions over the past decade. This major shift has led to the development of new HTS methodologies extending beyond the capabilities and target classes used in classical drug discovery approaches such as traditional enzymatic activity-based screens. In this brief review we describe some of the most interesting developments in HTS technologies and methods for chemical probe discovery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 30, February 2016, Pages 28–36
نویسندگان
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