کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1259360 1496497 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methods for the proteomic identification of protease substrates
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Methods for the proteomic identification of protease substrates
چکیده انگلیسی

Proteolysis is a key regulatory post-translational modification in diverse cellular processes including programed cell death, immune function, and development. Tracking proteolytic events has become a focus of researchers assessing the downstream consequences of protease activation. In this review we summarize unbiased methods for identifying protease substrates and tracking the extent of cleavage, a field termed ‘degradomics’. These include one-dimensional and two-dimensional gel-based methods for identifying protease substrates, N-terminal peptide identification methods for simultaneously identifying substrates and cleavage sites, and approaches for the quantitation of cleavage events during endogenous proteolysis. Individual methods have identified more than 300 caspase-cleaved targets during apoptosis suggesting broad future applications for these technologies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 13, Issues 5–6, December 2009, Pages 503–509
نویسندگان
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