Construction of a microbial natural product library for chemical biology studies

The RIKEN Natural Products Depository (NPDepo) is a public depository of small molecules. Currently, the NPDepo chemical library contains 39,200 pure compounds, half of which are natural products and their derivatives. In order to reinforce the uniqueness of our chemical library, we have improved our strategies for the collection of microbial natural products. Firstly, a microbial metabolite fraction library coupled with an MP (microbial products) plot database provides a powerful resource for the efficient isolation of microbial metabolites. Secondly, biosynthetic studies of microbial metabolites have enabled us to not only access ingenious biosynthetic machineries, but also obtain a variety of biosynthetic intermediates. Our chemical library contributes to the discovery of molecular probes for increasing our understanding of complex biological processes and for eventually developing new drug leads.

► NPDepo provides a chemical library that primarily focuses on natural products.
► A microbial metabolite fraction library with an MP plot database was constructed.
► The fraction library facilitates the collection of structurally unique metabolites.
► Genetically modified strains enable us to access biosynthetic intermediates.
► The microbial metabolites collected are applicable to screenings of bioactive compounds.