کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1267254 1496927 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical potential of Microgramma vaccinifolia rhizome lectin
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Electrochemical potential of Microgramma vaccinifolia rhizome lectin
چکیده انگلیسی

This work reports the isolation of Microgramma vaccinifolia rhizome lectin (MvRL) and the determination of electrochemical potentials of MvRL in the presence of Ca2 +, Mg2 + and human type O erythrocytes. MvRL showed the highest specific hemagglutinating activity with human type O erythrocytes and showed a single polypeptide band of 17 kDa on SDS-PAGE. MvRL hemagglutinating activity was neutralized after dialysis with EDTA, and addition of Ca2 + and Mg2 + restored the activity. Electrochemical potentials of MvRL in the presence of 100 mM Ca2 + (882 mV) and 60 mM Mg2 + (1051 mV) were higher (p < 0.05) than in the presence of only 0.15 M NaCl (247 mV), indicating that the electrochemical system was sensitive to structural and physico-chemical changes promoted by these ions. MvRL potential did not change in the presence of type O erythrocytes. The electrochemical system was able to detect changes in electrochemical potentials of MvRL promoted by Ca2 + and Mg2 +, even in a complex environment (human serum supplemented with 40 and 60 mM of these ions). The study reveals that the stimulatory effect of Ca2 + and Mg2 + on hemagglutinating activity may be linked to conformational change and/or alterations in surface charge distribution of MvRL.


► M. vaccinifolia rhizome lectin (MvRL) is a cation (Ca2 + and Mg2 +) dependent lectin.
► Electrochemical potentials of MvRL increased in the presence of Ca2 + and Mg2 +.
► The potential of MvRL did not change in the presence of human O erythrocytes.
► Increase in hemagglutinating activity may be due to changes in MvRL surface charge.
► The system worked even in the presence of human serum, a complex environment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 85, June 2012, Pages 56–60
نویسندگان
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