کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1272285 972554 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of anticancer herbal drug berberine with DNA immobilized on the glassy carbon electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Interaction of anticancer herbal drug berberine with DNA immobilized on the glassy carbon electrode
چکیده انگلیسی

The interaction of anticancer herbal drug berberine with double-strand DNA (dsDNA) and single-strand DNA (ssDNA) in solution, dsDNA immobilized on the glassy carbon electrode prepared by Langmuir–Blodgett technique, were investigated by electrochemical techniques (cyclic voltammetry, differential pulse voltammetry) and UV spectroscopy. The presence of DNA results in a decrease of the currents and a negative shift of the electrode potentials from the DPV curves of berberine, indicating the dominance of electrostatic interactions. The spectroscopy data confirmed that the predominant interaction between berberine and DNA is electrostatic. The binding of berberine with DNA, when analyzed in terms of the cooperative Hill model, yields the binding constant Ka = 2.2(± 0.2) × 104 M− 1, corresponding to the dissociation equilibrium constant Kd = 4.6(± 0.3) × 10− 5 M, which in the range of the applied concentrations of DNA (bp) and berberine, and a Hill coefficient m = 1.82(± 0.08) in Britton–Robinson buffer solution (0.05 M, pH 5.72) at T = 298 K (25 °C). Apparently, at least two molecules of berberine have to bind as a couple to cause, e.g., the “elementary event” of current change. The results are suggestive for further fruitful applications of this anticancer herbal drug and DNA-modified electrodes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 73, Issue 1, June 2008, Pages 18–22
نویسندگان
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