کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1272495 | 1496932 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
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چکیده انگلیسی
In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “phase diagram”, which delineates the boundaries for 1) effective EP of five different size molecules and 2) electric cell lysis at the single-cell level. In addition, these boundary curves (i.e., electric field versus pulse duration) were fitted successfully with an exponential function with three constants. We found that, when the molecular size increases, the corresponding electroporation boundary becomes closer to the electric cell lysis boundary. Based on more than 2000Â single-cell measurements on five different size molecules, the critical size of molecule was found to be approximately 40Â kDa. Comparing to the traditional instrument, MEMS-based micro electroporation chip can greatly shorten the experimental time.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 70, Issue 2, May 2007, Pages 363-368
Journal: Bioelectrochemistry - Volume 70, Issue 2, May 2007, Pages 363-368
نویسندگان
Huiqi He, Donald C. Chang, Yi-Kuen Lee,