کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1275735 | 1497537 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Isolation and characterization of a new hydrogen-producing strain Bacillus sp. FS2011 Isolation and characterization of a new hydrogen-producing strain Bacillus sp. FS2011](/preview/png/1275735.png)
A new fermentative hydrogen-producing strain FS2011 was isolated from an effluent of bio-hydrogen production reactor, and identified as Bacillus amyloliquefaciens on the basis of 16S rDNA gene sequence. The strain could utilize various carbon and nitrogen sources to produce hydrogen in a broad range of initial pH (5.29–7.38). Phosphate buffer concentration and fermentation temperature significantly affected hydrogen production and cell growth. The maximum hydrogen yield of 2.26 mol/mol was observed at glucose concentration of 10 g/l, beef extract concentration of 2 g/l, initial pH 6.98, phosphate buffer of 20 mmol/l, and 35 °C, indicating FS2011 was a high-efficiency hydrogen-producing bacterium.
► A new strain FS2011 was isolated and identified as Bacillus sp. by 16S rDNA.
► It could utilize various types of C and N sources to produce H2 at pH 5.3–7.4.
► Phosphate buffer and temperature importantly affected H2 yield and cell growth.
► The maximum H2 yield of 2.26 mol/mol occurred at 10 g/l glucose, pH 7.0 and 35 °C.
► The strain is a preponderant hydrogenogen compared with literature data.
Journal: International Journal of Hydrogen Energy - Volume 38, Issue 8, 19 March 2013, Pages 3206–3212