Escherichia coli (XL1-BLUE) for continuous fermentation of bioH2 and its separation by polyimide membrane

In this work continuous hydrogen production by Escherichia coli (XL1-BLUE) and its purification by membrane gas separation were studied. Firstly, a kinetic investigation was performed on formate supplemented broth in order to determine exponential growth phase (5–7 h) while the most intense hydrogen fermentation takes place. Furthermore, important process design parameters such as saturation constant and maximal growth rate were calculated (KS = 0.77 g l−1, μmax = 0.39 h−1). Afterward, based on the kinetic study, continuous hydrogen fermentations using cultures of E. coli (XL1-BLUE) were carried out in a CSTR reactor configuration applying various hydraulic retention times (HRT) related to both exponential and stationary growth period (5 h, 7 h, 9 h). The results indicated that highest hydrogen yield (0.26 mmol H2/mmol formate added) and productivity (5.1 mmol H2 l−1 d−1) could be achieved by applying HRT = 7 h that does not allow the living cells to reach stationary phase. In addition to hydrogen production, the concentration of bioH2 by polyimide membrane under different operational circumstances was investigated using pure and mixed gases, as well. The results of single gas experiments indicated that increasing the temperature has positive effect on separation efficiency. Moreover, the influence of retentate and feed flow ratio (QR/QF) was studied applying binary H2/CO2 gaseous mixture and it was found that polyimide membrane has high potential for H2 purification since 18% increase in H2 concentration and 22% decrease in CO2 content could be attained in the permeate by a one-step separation process.

► Study on bacterial growth kinetic using Escherichia coli (XL1-BLUE).
► Determination of exponential growth phase and kinetic constants.
► Continuous hydrogen fermentation in CSTR applying various HRTs based on kinetic investigation.
► Hydrogen purification by polyimide gas separation membrane.