کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1279979 1497664 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative monitoring of a hydrogen-producing Clostridium butyricum strain from a continuous-flow, mixed culture bioreactor employing real-time PCR
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Quantitative monitoring of a hydrogen-producing Clostridium butyricum strain from a continuous-flow, mixed culture bioreactor employing real-time PCR
چکیده انگلیسی

A quantitative real-time PCR (qrt-PCR) method was developed for quantification of a hydrogen-producing Clostridium butyricum   strain. The strain was the main hydrogen producer in a mixed culture bioreactor that was operated on a continuous-flow mode for 156 days. The qrt-PCR target was a segment of 16S rDNA, which was amplified from DNA extracted from bioreactor samples. The amplified target was quantified by measuring lanthanide fluorescence in a time-resolved manner. The linear range of this qrt-PCR assay was 102–107102–107 bacterial genomes. The results showed substantial variation (over two orders of magnitude) in the quantity of the target 16S rDNA of C. butyricum during the continuous-flow operation. Hydrogen production rate and butyrate concentration also varied with time. The method is applicable to any selected species in bioreactors and provides valuable insight into bioprocess performance and microbial community structure in complex dynamic systems.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 33, Issue 2, January 2008, Pages 542–549
نویسندگان
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