کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1280250 | 1497599 | 2010 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Improvement of hydrogen yield by hupR gene knock-out and nifA gene overexpression in Rhodobacter sphaeroides 6016 Improvement of hydrogen yield by hupR gene knock-out and nifA gene overexpression in Rhodobacter sphaeroides 6016](/preview/png/1280250.png)
Purple non-sulfur (PNS) bacteria produce hydrogen under photoheterotrophic conditions. The produced hydrogen, which is catalyzed by Mo-nitrogenase, is re-oxidized by a membrane-bound uptake hydrogenase. To improve hydrogen-producing efficiency, the hupSL gene that encodes the uptake hydrogenase, and its positive regulator gene hupR were knocked out in Rhodobacter sphaeroides 6016. Compared to the wild-type strain, the hydrogen yields of the hupSL− and hupR− mutants each increased by 11%. When the nifA gene, which encodes a specific transcriptional activator for all other nif genes was overexpressed in the hupR− mutant, the hydrogen yield increased by 20%. In addition, hydrogen production was detected in the nifA/hupR− mutant in the presence of 14 mM NH4+, whereas the wild-type strain produced no hydrogen under the same conditions.
Journal: International Journal of Hydrogen Energy - Volume 35, Issue 18, September 2010, Pages 9603–9610