کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1280817 | 1497694 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of changes in the level of light harvesting complexes of Rhodobacter sphaeroides on the photoheterotrophic production of hydrogen Effect of changes in the level of light harvesting complexes of Rhodobacter sphaeroides on the photoheterotrophic production of hydrogen](/preview/png/1280817.png)
Increase in levels of photosynthetic spectral complexes by maintaining the plasmids harboring DNA encoding puhA, pufBA, or pucBAC in trans in Rhodobacter sphaeroides resulted in decrease of the photoheterotrophic production of H2. However, removal of B875 or B800–850 light-harvesting (LH) complexes affected H2 production differently. Lack of B875 complex following in-frame deletion of pufBA (mutant PUF1) not only slowed photoheterotrophic growth but also decreased H2 production, indicative of the essential requirement of the complex for LH process. However, the pucBA-deleted mutant, PUC1 lacking of B800–850 complex, increased H2 production in comparison with its parental cell by approximately twofold, given irradiated with light (10 W/m2) saturating the growth of wild type. The H2 production of PUC1 did not increase in proportion to the light intensity, which is also observed with wild type. Thus, we suggest that light is not limited for the H2 production of the cells under the experimental conditions employed in this work, but the cellular energy to be used for the formation of B800–850 complex may flow into the metabolism leading to the H2 production in PUC1.
Journal: International Journal of Hydrogen Energy - Volume 31, Issue 4, March 2006, Pages 531–538