کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1281186 | 1497636 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Relationship between molecular hydrogen production, proton transport and the F0F1-ATPase activity in Rhodobacter sphaeroides strains from mineral springs Relationship between molecular hydrogen production, proton transport and the F0F1-ATPase activity in Rhodobacter sphaeroides strains from mineral springs](/preview/png/1281186.png)
This article aims to study hydrogen production and proton transport in two strains of purple non-sulfur bacterium Rhodobacter sphaeroides isolated from mineral springs of Armenia. This bacterium is able to grow and produce molecular hydrogen (H2) in anaerobic conditions upon illumination. Along with H2 production, a marked decrease in redox potential and the alkalization of the medium have been observed; the latter might be the evidence of proton influx. H2 production and alkalization of the medium by whole cells both are suppressed by the F0F1-ATPase inhibitors – N,N′-dicyclohexylcarbodiimide (DCCD), sodium azide (NaN3) and protonophore – carbonyl cyanide m-chlorophenylhydrazone (CCCP). Membrane vesicles of two strains of R. sphaeroides demonstrate ATPase activity, inhibited by DCCD and NaN3, but not by CCCP. These results indicate a relationship between H2 production, proton transport and the F0F1-ATPase activity that might be a pathway to regulate bacterial activity under anaerobic conditions.
Journal: International Journal of Hydrogen Energy - Volume 34, Issue 6, March 2009, Pages 2567–2572