کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1282275 | 1497551 | 2012 | 7 صفحه PDF | دانلود رایگان |

In this work, the kinetic characterization of hydrogen production by the photofermentative bacteria Rhodopseudomonas faecalis RLD-53 was investigated at different growth phase. During entire fermentation, 89.30% of total biomass was accumulated in exponential growth phase, while hydrogen yield was only 1.82 mol H2/mol acetate at the expense of 51.25% substrate. In the stationary phase, biomass synthesis was minimal (7.51%), and 38.17% of the substrate was directly converted into hydrogen. As a result, hydrogen (59.19%) was mainly produced in stationary phase with highest hydrogen yield of 3.67 mol H2/mol acetate. Consequently, bacteria in stationary phase were most effective for hydrogen production. Based on these findings, a novel membrane photobioreactor was developed to retain bacteria during stationary phase in reactor through membrane separation. Maximum rate (32.82 ml/l/h) and yield (3.27 mol H2/mol acetate) of hydrogen production were achieved using membrane photobioreactor under the continuous operation. Therefore, using bacteria in stationary phase as hydrogen producer can offer considerable benefits for enhancing photo-hydrogen production.
► Hydrogen production rate had a linear relationship with nitrogenase activity.
► Bacteria in stationary phase were most effective for photo-hydrogen production.
► Membrane photobioreactor was developed in line with characteristics of bacteria.
► Bacteria in stationary phase were retained in reactor through membrane separation.
► Using bacteria in stationary phase can offer considerable benefits for H2 production.
Journal: International Journal of Hydrogen Energy - Volume 37, Issue 18, September 2012, Pages 13718–13724