کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1310885 | 1499200 | 2012 | 8 صفحه PDF | دانلود رایگان |
Two new ligands APIP, HAPIP and their relative ruthenium(II) complexes [Ru(bpy)2(APIP)](ClO4)21 and [Ru(bpy)2(HAPIP)](ClO4)22 have been synthesized and characterized. The DNA binding constants for complexes 1 and 2 have been determined to be 6.08 (±0.29) × 104 M−1 and 1.78 (±0.30) × 105 M−1. The results suggest that these complexes intercalate between the base pairs of DNA. The cytotoxicity of complexes has been evaluated by MTT assay. The apoptosis assay was carried out with AO/EB staining methods. The cellular uptake was observed under fluorescence microscopy. The studies on the mechanism of photocleavage demonstrate that superoxide anion radical (O2−) and singlet oxygen (1O2) may play an important role. The antioxidant activity against hydroxyl radical (OH) was also studied.
Two new ruthenium(II) complexes [Ru(bpy)2(APIP)]2+ (1) and [Ru(bpy)2(HAPIP)]2+ (2) have been synthesized and characterized. Their cytotoxicity, apoptosis, cellular uptake, DNA-binding and antioxidant activity against hydroxyl radical have been studied.Figure optionsDownload as PowerPoint slideHighlights
► Two ruthenium(II) complexes were synthesized and characterized.
► The DNA-binding behaviors were investigated.
► The cytotoxicity of these complexes has been evaluated by MTT assay.
► The apoptosis assay was carried out with AO/EB staining methods.
► The cellular uptake was observed under fluorescence microscopy.
Journal: Inorganica Chimica Acta - Volume 387, 30 May 2012, Pages 117–124