کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1316103 976424 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Insect cytochromes P450: Topology of structural elements predicted to govern catalytic versatility
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Insect cytochromes P450: Topology of structural elements predicted to govern catalytic versatility
چکیده انگلیسی

Among eukaryotic P450s, the greatest expansion has been in insects, providing useful model systems for the study of enzyme evolution in response to natural and anthropogenic pressures such as the chemical warfare against plant toxins and synthetic insecticides. To better understand diversification of the catalytic properties in the various P450 clades, insight into the molecular principles governing biotransformation of the array of endogenous and exogenous compounds is of paramount importance. Based on a general, CYP102A1-related construct, the majority of prospective substrate-docking residues were found to cluster near the distal heme face within the six known substrate recognition sites (SRSs) made up by the α-helical B′, F, G and I tetrad as well as the B′-C turn and strands of certain β-sheets. Highest proportion of contact sites, having a mean frequency of conservation of not more than 10%, was detected to be housed in the variable SRS-1 and SRS-2 domains, affording conformational mobility to meet the structural diversity in the bulk of substrates. Some key determinants may have a function in governing substrate access, positioning ligands toward the catalytic center to allow regioselective attack, and promoting dioxygen activation. Moreover, non-SRS elements occupying regions on the bowl-like proximal heme face may serve in recognition of electron delivery partners. Physico-chemical factors interfering with substrate anchoring mainly relate to the variably expressed lipophilicity/hydrophilicity profile of the diverse binding sites and bulkiness of the side chains of critical amino acids. Detailed knowledge about structure–function relationships in insect P450s, thus, may offer an incentive to tailoring of novel insecticides and synergistic enzyme inhibitors to more efficiently control insect pests.

The three-dimensional topographic map reveals the majority of contact points predicted to dictate substrate turnover to cluster in α-helical and β-sheet structures close to the heme face, while sites located on the enzyme's proximal surface mainly serve in redox partner docking.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 105, Issue 10, October 2011, Pages 1354–1364
نویسندگان
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